تويت
رد: د (جيف برادستريت) وعلاج التوحد ب gc-maf .
في البحث في نهايه هذه الصفحه ريم توضيح لطريقه تحضير الGCMAF
تجدي هذا الجزء تحت عنوان Mono Mac 6 cells as a tool to test GcMAF-like activity
Thus, we tested a raw preparation obtained in our laboratory, putatively containing GcMAF, on MM6 cell proliferation. This preparation was obtained treating colostrum with kefir grains. It is known that kefir modulates the immune response in mice, increasing the phagocytic activity (i.e. activating) of peritoneal and pulmonary macrophages (Immunobiology 2006; 211:149-56). It is conceivable that microorganisms of kefir grains could convert milk Gc protein into active GcMAF due to their enzymatic activities. We used colostrum instead of milk because colostrum is richer in Gc protein (J Nutr Biochem 1992; 3:498-502). Freshly collected colostrum was incubated for 24 h with kefir grains. The liquid supernatant was collected, filtered and incubated with MM6 cells for 72 h as in the experiments reported in Figs 1 and 2. Increasing concentration of kefir supernatant (expressed as total protein content, i.e. 4.8-480 ug/ml. Fig 3, columns 5-7) significantly inhibited MM6 proliferation in a manner similar to that observed with GcMAF.
في البحث في نهايه هذه الصفحه ريم توضيح لطريقه تحضير الGCMAF
تجدي هذا الجزء تحت عنوان Mono Mac 6 cells as a tool to test GcMAF-like activity
Thus, we tested a raw preparation obtained in our laboratory, putatively containing GcMAF, on MM6 cell proliferation. This preparation was obtained treating colostrum with kefir grains. It is known that kefir modulates the immune response in mice, increasing the phagocytic activity (i.e. activating) of peritoneal and pulmonary macrophages (Immunobiology 2006; 211:149-56). It is conceivable that microorganisms of kefir grains could convert milk Gc protein into active GcMAF due to their enzymatic activities. We used colostrum instead of milk because colostrum is richer in Gc protein (J Nutr Biochem 1992; 3:498-502). Freshly collected colostrum was incubated for 24 h with kefir grains. The liquid supernatant was collected, filtered and incubated with MM6 cells for 72 h as in the experiments reported in Figs 1 and 2. Increasing concentration of kefir supernatant (expressed as total protein content, i.e. 4.8-480 ug/ml. Fig 3, columns 5-7) significantly inhibited MM6 proliferation in a manner similar to that observed with GcMAF.
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